Epi Laser illumination Profiles

Many microscope laser illumination techniques involve the use of a beamsplitter or a fluorescent filter cube to direct illumination to the objective. The CaliCube can replace these components, providing an image of the laser illumination system, as seem in the conjugate plane of  the microscope to the microscope imaging system. This enables the CaliCube to provide users with an image similar to a laser beam profiler. These images can be used to trouble shoot or evaluate the performance of of these illumination systems as they perform inside the microscope. Laser beam characteristics include Beam Diameter, Beam Quality, Beam Divergence, Beam Wander or Jitter. They are all measurable quantities that a properly configured video camera in the light path of a laser can provide. The CaliCube can be ordered with custom filters to best suite it's performance in a laser environment. It can also be ordered to fit into a beamsplitter turret or slider as shown below. Please contact Tom at Epi Technology to discuss details.  

  Image 1 shows two images microscopes configured with Confocal Illumination. In this example, a normal beamsplitter reflects the confocal illumination into the objective optical axis. The second image shows a CaliCube in place of a second beamsplitter that diverts the laser illumination directly to the microscope imaging system, enabling the imaging system to perform the functions of a beam profiler. These images can be taken a regular intervals to ensure laser performance is optimal. 

Image 1 shows two images microscopes configured with Confocal Illumination. In this example, a normal beamsplitter reflects the confocal illumination into the objective optical axis. The second image shows a CaliCube in place of a second beamsplitter that diverts the laser illumination directly to the microscope imaging system, enabling the imaging system to perform the functions of a beam profiler. These images can be taken a regular intervals to ensure laser performance is optimal. 

  Image 2 shows two images of the same confocal microscope, highlighting the functions of the conventionally illuminated  epi widefield light path. The image on the right shows a CaliCube in place of a normal fluorescence filter cube, relaying a diagnostic image of the light source as it appears in illumination plane of the microscope. 

Image 2 shows two images of the same confocal microscope, highlighting the functions of the conventionally illuminated  epi widefield light path. The image on the right shows a CaliCube in place of a normal fluorescence filter cube, relaying a diagnostic image of the light source as it appears in illumination plane of the microscope. 

  Image 3 shows an inverted microscope with a confocal attachment. The image on the right shows a custom CaliCube installation on the prism relay turret at the bottom of the microscope, relaying a diagnostic image to an imaging camera installed on the bottom port of the microscope.

Image 3 shows an inverted microscope with a confocal attachment. The image on the right shows a custom CaliCube installation on the prism relay turret at the bottom of the microscope, relaying a diagnostic image to an imaging camera installed on the bottom port of the microscope.